Let us begin our journey... Just to share with you my plan for the animal work.
Insya-allah after this eid fitr holiday, we'll start our animal work until a few weeks. Roughly, every monday begin from 13-10-08, i'll take tha rats from animal house (7 rats). Oh ya, forgot to write a letter to Prof Dr Rais, Head of Pharmacology Dept.
Why do i need to write a letter to him? Because i want to put my rat at his department animal room. At the same time, i'll be using his plethysmometer.
After that, i'll take the rat's blood from the tail. Remember to prepare hot water around 60 degrees, as what was advice by our expert, dedicated and well experienced medical lab technologist, Mr Linggam from Dept of Physiolgy. According to him, i can around 2 ml blood. So, roughly i can 1 ml serum to be use for biochemical analysis, insya-allah.
I'm wondering, do i need to measure the rat body weight? Yes, because i need to know the volume for injection. How to calculate the relation and association between the the rat body weight and volume of injection? Wait for next entry ya, insya-allah.
So, after withdraw the blood and put in plain tube, i need to left it at room temperature for 30 minutes before centrifuge to get the serum.
How long to centrifudge? How many rpm?
Wait until the next post ya!
Selamat berbuka puasa! Dont forget to make doa for me because the time near breaking fast is the time which doa is 'makbul'. This was guaranteed by out beloved Prophet from his saying.
Subscribe to:
Post Comments (Atom)
No comments:
Post a Comment