My undergraduate reseach project..from http://robert2007.blog.com/?page=3
i still remembered, this news was published in Harian Metro but unfortunately, did't keep it as my personal copy...Enjoy your reading!
Sabun cantikkan kulit
KELEBIHAN dan keberkesanan yang ada pada sabun susu kambing menjadikan produk itu pilihan kebanyakan masyarakat hari ini. Ia bukan saja kaya dengan mineral serta protein, tetapi mengandungi pelbagai vitamin berguna sebagai pencuci dan pelembab paling mujarab untuk kulit.
Ini kerana susu kambing mempunyai kandungan lemak tinggi jika dibandingkan dengan susu lembu. Selain itu, ia mengandungi asid lemak yang penting (essential fatty acids) seperti linoleic dan arachidonic asid yang diperlukan badan.
Ia juga kaya dengan kalsium, vitamin B-6, vitamin A, potassium dan selenium.
Hari ini, ada pelbagai produk sabun susu kambing hasil campuran susu kambing dengan bahan lain, seperti herba atau Aloe Vera, untuk memperolehi kesan lebih baik.
Bagaimanapun, kini penyelidik dari Universiti Malaya (UM) berjaya menghasilkan sabun susu kambing campuran herba dan dadih - dipercayai yang pertama dihasilkan di negara ini.
Sabun yang dinamakan Sabun Herba Yoghurt Susu Kambing (Soaphy) itu berkhasiat untuk memutihkan kulit, menyingkirkan sel mati, mengatasi masalah seperti kulit kering, ruam, jerawat juga membersih dan melembapkan kulit.
Menurut Ketua Penyelidik yang mencipta sabun berkenaan, Profesor Madya Dr Ahmad Salihin Baba, campuran ketiga-tiga bahan itu berupaya mendapatkan hasil lebih baik dengan gabungan kelebihan setiap bahan.
Katanya, Soaphy mengandungi 30 peratus susu kambing sejati yang dicampurkan dengan herba seperti daun jambu batu (guajava), kayu manis (cinnamon), bunga lawang (verum), lada hitam (nigrum) atau herba cina (japonica) dan sedikit dadih.
Selain campuran itu, Soaphy turut dihasilkan daripada ramuan berkualiti tinggi, antaranya minyak kelapa sawit, minyak kelapa dan wangian pati tumbuhan.
Campuran
"Soaphy tidak mengandungi pewarna atau pengawet tiruan, begitu juga susu kambing tidak dicampur air dan susu tepung.
"Dengan bahan berkualiti serta dengan campuran betul, didapati kandungan asid organiknya bertambah, sekaligus berpotensi meningkatkan kecerahan kulit berbanding biasa," katanya ketika ditemui pada Ekspo Penyelidikan dan Inovasi UM 2004, baru-baru ini.
Beliau yang juga pensyarah di Bahagian Biokimia, Institut Sains Biologi, Fakulti Sains UM berkata, herba menjadi pilihannya kerana ia memang diakui berkhasiat seperti melancarkan perjalanan darah, awet muda serta menambah selera makan.
Menurutnya, dadih pula mengandungi sejenis bakteria, probiotik, yang berupaya mengatasi masalah penghadaman susu.
Katanya, dadih juga berupaya mengeluarkan sejenis enzim, beta-galaktosidage yang boleh menghuraikan laktosa kepada dua bahagian, iaitu glukosa dan galaktisa.
"Beta-galaktosidage akan menghuraikan laktosa menjadi glukosa dan galaktisa, yang mana kedua-duanya boleh menjadi sumber tenaga kepada seseorang apabila meminumnya," katanya.
Ahli kumpulan, Mohd Nuruddin Abd Manap, 22, berkata melalui kajian makmal, mereka mendapati kandungan anti-oksida dan anti-mutagen Soaphy adalah tinggi, sekaligus boleh mengelakkan kanser berlaku.
"Mutagen adalah ejen yang menyebabkan mutasi berlaku. Proses mutasi ini, iaitu pembahagian sel tanpa kawal, boleh menyebabkan perubahan kepada DNA (deoxyribonucleic acid) yang menjadi punca wujudnya kanser.
"Selain itu, sabun ini berkesan sebagai aromaterapi kerana baunya wangi disebabkan penggunaan wangian pati tumbuhan. Jadi ia selamat, lembut serta mesra kepada alam sekitar mahupun penggunanya," kata pelajar tahun akhir Bio Kesihatan Institut Sains Biologi, Fakulti Sains UM itu.
Selain Mohd Nuruddin, ahli kumpulan lain yang menghasilkan Soaphy itu ialah Khairul Bariah Abu Hassan Asshaari dan Aidah Shamsuddin.
Menurutnya, kajian untuk menghasilkan Soaphy sejak empat tahun lalu itu mendapat bantuan Majlis Kanser Nasional (Makna) dengan tujuan membantu mereka yang memerlukan.
Thursday, January 29, 2009
Monday, January 12, 2009
How True is this news?
After a long time i didn't update this blog, alhamdulillah..finally i was given a 'petunjuk' to update this blog.. Yes, there are many 'petunjuk' that we really need in our daily life. In fact, updating a blog need a 'petunjuk' if we really sincere to use this platform to do something for the benefit of mankind.
For your information, i got this news from apitherapy blog. You can access through this link http://apitherapy.blogspot.com/2007/06/bee-venom-therapy-popular-in-malaysia.html
When i read the title, honestly, i feel very suspicious. How true is this news? Why? If we really want to go to the place that offered the bee venom therapy as mentioned in the news, where should we go? Where is the location? In fact, this news was published in NST. So, enjoy your reading and don't forget to think about that!
Bee Venom Therapy Popular in Malaysia
Just a Sting Away From Pain Relief
By Sim Bak Heng, New Straits Times (Malaysia), 6/15/2007
Most of us duck and run when it comes to bees, but there are people who look forward to a sting or two, or three, or more. These people are undergoing bee venom therapy, which has become a popular alternative to acupuncture for a host of painful conditions including arthritis, rheumatism and gout, asthma and pre-menstrual tension.
Some proponents claim the therapy also relieves male impotence, the symptoms of psoriasis, epilepsy and depression, and the pain of cancer…
The owner of one bee farm says it is important for clients to be tested first to check the reaction to the venom. A allergic reaction can be fatal.
Yazid Mohamad, who has had arthritis for years, goes to a bee farm for treatment.
"I read about bee venom therapy and tried it on my own. It works for me, and I have introduced it to many of my friends," he said.
"The stings are much less painful than a jab. The venom injected into my knees and finger joints relieves my pain within 15 minutes and lasts for at least a day."
Bee venom contains more than 40 active substances, including the protein melittin, which stimulates cortisol production by the adrenal glands, which is a natural anti-inflammatory.
The venom also contains "Peptide 401" which is believed to be 100 times more powerful than synthetic cortico-steroids…
For your information, i got this news from apitherapy blog. You can access through this link http://apitherapy.blogspot.com/2007/06/bee-venom-therapy-popular-in-malaysia.html
When i read the title, honestly, i feel very suspicious. How true is this news? Why? If we really want to go to the place that offered the bee venom therapy as mentioned in the news, where should we go? Where is the location? In fact, this news was published in NST. So, enjoy your reading and don't forget to think about that!
Bee Venom Therapy Popular in Malaysia
Just a Sting Away From Pain Relief
By Sim Bak Heng, New Straits Times (Malaysia), 6/15/2007
Most of us duck and run when it comes to bees, but there are people who look forward to a sting or two, or three, or more. These people are undergoing bee venom therapy, which has become a popular alternative to acupuncture for a host of painful conditions including arthritis, rheumatism and gout, asthma and pre-menstrual tension.
Some proponents claim the therapy also relieves male impotence, the symptoms of psoriasis, epilepsy and depression, and the pain of cancer…
The owner of one bee farm says it is important for clients to be tested first to check the reaction to the venom. A allergic reaction can be fatal.
Yazid Mohamad, who has had arthritis for years, goes to a bee farm for treatment.
"I read about bee venom therapy and tried it on my own. It works for me, and I have introduced it to many of my friends," he said.
"The stings are much less painful than a jab. The venom injected into my knees and finger joints relieves my pain within 15 minutes and lasts for at least a day."
Bee venom contains more than 40 active substances, including the protein melittin, which stimulates cortisol production by the adrenal glands, which is a natural anti-inflammatory.
The venom also contains "Peptide 401" which is believed to be 100 times more powerful than synthetic cortico-steroids…
Thursday, October 9, 2008
Only 23% of Honey Sold in Malaysia is Pure?
Abstracts from the 1st International Conference on the Medicinal Uses of Honey (From Hive to Therapy) held by Universiti Sains Malaysia in August of 2006. The abstracts have been published by the Malaysian Journal of Medical Sciences (Vol.14 No.1, January 2007).
http://www.medic.usm.my/publication/mjms/our_journal/mjms_january2007/Honey_Conf_Abstract.pdf
Wednesday, September 17, 2008
Begin a journey....
Let us begin our journey... Just to share with you my plan for the animal work.
Insya-allah after this eid fitr holiday, we'll start our animal work until a few weeks. Roughly, every monday begin from 13-10-08, i'll take tha rats from animal house (7 rats). Oh ya, forgot to write a letter to Prof Dr Rais, Head of Pharmacology Dept.
Why do i need to write a letter to him? Because i want to put my rat at his department animal room. At the same time, i'll be using his plethysmometer.
After that, i'll take the rat's blood from the tail. Remember to prepare hot water around 60 degrees, as what was advice by our expert, dedicated and well experienced medical lab technologist, Mr Linggam from Dept of Physiolgy. According to him, i can around 2 ml blood. So, roughly i can 1 ml serum to be use for biochemical analysis, insya-allah.
I'm wondering, do i need to measure the rat body weight? Yes, because i need to know the volume for injection. How to calculate the relation and association between the the rat body weight and volume of injection? Wait for next entry ya, insya-allah.
So, after withdraw the blood and put in plain tube, i need to left it at room temperature for 30 minutes before centrifuge to get the serum.
How long to centrifudge? How many rpm?
Wait until the next post ya!
Selamat berbuka puasa! Dont forget to make doa for me because the time near breaking fast is the time which doa is 'makbul'. This was guaranteed by out beloved Prophet from his saying.
Insya-allah after this eid fitr holiday, we'll start our animal work until a few weeks. Roughly, every monday begin from 13-10-08, i'll take tha rats from animal house (7 rats). Oh ya, forgot to write a letter to Prof Dr Rais, Head of Pharmacology Dept.
Why do i need to write a letter to him? Because i want to put my rat at his department animal room. At the same time, i'll be using his plethysmometer.
After that, i'll take the rat's blood from the tail. Remember to prepare hot water around 60 degrees, as what was advice by our expert, dedicated and well experienced medical lab technologist, Mr Linggam from Dept of Physiolgy. According to him, i can around 2 ml blood. So, roughly i can 1 ml serum to be use for biochemical analysis, insya-allah.
I'm wondering, do i need to measure the rat body weight? Yes, because i need to know the volume for injection. How to calculate the relation and association between the the rat body weight and volume of injection? Wait for next entry ya, insya-allah.
So, after withdraw the blood and put in plain tube, i need to left it at room temperature for 30 minutes before centrifuge to get the serum.
How long to centrifudge? How many rpm?
Wait until the next post ya!
Selamat berbuka puasa! Dont forget to make doa for me because the time near breaking fast is the time which doa is 'makbul'. This was guaranteed by out beloved Prophet from his saying.
How to prepare Carrageenan?
Before that, what's is carrageenan (CR)?
Why we need carrageenan?
Som what;s your answer?
In my study, i need to use 1% carrageenan.
Let us explore how to prepare for it;
1 g CR dissolved in 100 ml deionised water
So, 1000 mg CR = 100 ml deionised water
10 mg CR = 1 ml deionised water
Easy right? Is it enough for our study?
We just need 100 microliter per rat to inject in the subplantar area of the rat hind paw.
So, do you think it's enough?
Why we need carrageenan?
Som what;s your answer?
In my study, i need to use 1% carrageenan.
Let us explore how to prepare for it;
1 g CR dissolved in 100 ml deionised water
So, 1000 mg CR = 100 ml deionised water
10 mg CR = 1 ml deionised water
Easy right? Is it enough for our study?
We just need 100 microliter per rat to inject in the subplantar area of the rat hind paw.
So, do you think it's enough?
How to prepare Indomethacine Drug?
Dose of indomethacine = 10 mg/Kg
How to prepare?
10 mg = 1 kg = 1000 g
So, 1 mg = 100 g
If the rat body weight is 200 g, we need 2 mg of indomethacine
If i want to inject 100 microliter for each rat, perhaps i have 7 rats, s0;
2 mg x 7 = 14 mg = 700 microliter which 100 microliter for each rat
Better to prepare extra;
2 mg x 10 = 20 mg = 1 ml
As a conclusion, 20 mg of indomethacine will be dissolved in 1 ml 0.5% Sodium Bicarbonate
Now, let us see how 0.5% Sodium Bicarbonate was prepared;
0.5 g of Sodium Bicarbonate dissolved in 100 ml deionised water
0.05 g of Sodium Bicarbonate dissolved in 10 ml deionised water
0.025g of Sodium Bicarbonate dissolved in 5 ml deionised water
Easy right?
Now, you can try to prepare the same dose of indomethacine for the rat with body weight 300 g.
What's the answer?
How to prepare?
10 mg = 1 kg = 1000 g
So, 1 mg = 100 g
If the rat body weight is 200 g, we need 2 mg of indomethacine
If i want to inject 100 microliter for each rat, perhaps i have 7 rats, s0;
2 mg x 7 = 14 mg = 700 microliter which 100 microliter for each rat
Better to prepare extra;
2 mg x 10 = 20 mg = 1 ml
As a conclusion, 20 mg of indomethacine will be dissolved in 1 ml 0.5% Sodium Bicarbonate
Now, let us see how 0.5% Sodium Bicarbonate was prepared;
0.5 g of Sodium Bicarbonate dissolved in 100 ml deionised water
0.05 g of Sodium Bicarbonate dissolved in 10 ml deionised water
0.025g of Sodium Bicarbonate dissolved in 5 ml deionised water
Easy right?
Now, you can try to prepare the same dose of indomethacine for the rat with body weight 300 g.
What's the answer?
Thursday, September 4, 2008
BV anti-inflammatory effect via inhibition of p-50 subunit of NF-kB
Title:
Antiarthritic effect of bee venom: inhibition of inflammation mediator generation by suppression of NF-kappaB through interaction with the p50 subunit.
Abstract available online via;
http://www.ncbi.nlm.nih.gov/pubmed/15529353
Method:
a) Anti-arthritic effect of BV in rat model;
1) carrageenan (CR) induced acute edema in the paw
2) chronic adjuvant-induced arthritis
b) Gene expression (inducible nitric oxide synthetase (iNOS), cyclooxygenase-2 (COX-2), cytosolic phospholipase A2 (cPLA2), and tumor necrosis factor-α (TNF-α)) measured by Western blotting
c) PGE2, NO and intracellular calcium (involved in the generation of superoxide anion, NO, TNF-α and iNOS)
d) p-50 subunit of NF-kB DNA determined by surface plasmon resonance analyzer
Antiarthritic effect of bee venom: inhibition of inflammation mediator generation by suppression of NF-kappaB through interaction with the p50 subunit.
Abstract available online via;
http://www.ncbi.nlm.nih.gov/pubmed/15529353
Method:
a) Anti-arthritic effect of BV in rat model;
1) carrageenan (CR) induced acute edema in the paw
2) chronic adjuvant-induced arthritis
b) Gene expression (inducible nitric oxide synthetase (iNOS), cyclooxygenase-2 (COX-2), cytosolic phospholipase A2 (cPLA2), and tumor necrosis factor-α (TNF-α)) measured by Western blotting
c) PGE2, NO and intracellular calcium (involved in the generation of superoxide anion, NO, TNF-α and iNOS)
d) p-50 subunit of NF-kB DNA determined by surface plasmon resonance analyzer
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